Journal: Aging and Disease

Article Title: Anillin Recedes in p53-Dependent Senescence of Tumor Cells and Reappears in Cells Escaping from Senescence

doi: 10.14336/AD.2025.0402

Figure Lengend Snippet: Analysis of the selected senescence markers and anillin levels in HCT116 p53WT and MCF-7 cells induced to senesce by 1 day-treatment with doxorubicin and collected 5 days after senescence induction. ( A, B ) Densitometric analysis of protein levels in HCT116 p53WT ( A ) and MCF-7 ( B ), n=9; statistical analysis was performed using paired one-tailed t-Student test. Relative protein expression means fold change (in the expression of proteins relative to the expression of GAPDH) vs appropriate control. Boxes: Q1, median, Q3; error bars: Minimum, Maximum. ( C ) Representative images from Western blotting of HCT116 p53WT and MCF-7 cell lysates. ( D, E ) Analysis of fluorescence intensity of anillin (whole nucleus area) and representative images ( F, G ) of control and doxorubicin-treated HCT116 p53WT ( D, F ) and MCF-7 cells ( E, G ), n=3; statistical analysis was performed using Wilcoxon matched-pairs signed-rank test. Data on graphs represent individual values for analyzed cells, median, error bars: Minimum, Maximum. Red – anillin, blue – DAPI stained DNA. Scale 20 µm. Statistical significance relative to control: ** p ≤ 0.01, *** p ≤ 0.001, **** p ≤ 0.0001

Article Snippet: The human HCT116 p53-proficient (referred to as HCT116 p53WT) colon cancer cell line and the breast cancer cell line MCF-7 were obtained from ATCC (HCT116 CCL-247; MCF-7 HTB-22).

Techniques: One-tailed Test, Expressing, Control, Western Blot, Fluorescence, Staining

Journal: Aging and Disease

Article Title: Anillin Recedes in p53-Dependent Senescence of Tumor Cells and Reappears in Cells Escaping from Senescence

doi: 10.14336/AD.2025.0402

Figure Lengend Snippet: Correlation between p53 and anillin levels during senescence and the escape from senescence in breast cancer MCF-7 and colon cancer HCT116 p53WT cells (see ). ( A-B ). Representative Western blots showing the levels of anillin and p53 in HCT116 p53WT cells ( A ) and MCF-7 cells ( B ). ( C-F ) The level of anillin and p53 on subsequent days of cell culture after senescence induction by doxorubicin in HCT116 p53WT ( C and E ) and MCF-7 cells ( D and F ) n = 4; statistical analysis was performed using one-way ANOVA followed by post hoc analysis (Tukey’s honest significant difference test; HSD test). Statistical significance of differences between indicated days of treatment: * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001. Boxes: Q1, median, Q3; error bars: Minimum, Maximum. ( G ) The heat map shows the levels of anillin and p53 during senescence and escape from senescence in MCF-7 and HCT116 p53WT cells. Heatmap: Original data points are standardized into z-scores.

Article Snippet: The human HCT116 p53-proficient (referred to as HCT116 p53WT) colon cancer cell line and the breast cancer cell line MCF-7 were obtained from ATCC (HCT116 CCL-247; MCF-7 HTB-22).

Techniques: Western Blot, Cell Culture

Journal: Aging and Disease

Article Title: Postbiotic Parabacteroides Distasonis Supplementation Enhances Intestinal and Skeletal Muscle Function in Aged Mice

doi: 10.14336/AD.2025.0188

Figure Lengend Snippet: Postbiotic Pd modifies mitochondria in a colon cell line. (A) Right panel-representative images of HCT116 treated or not with postbiotic Pd for 24h and labeled with TMRE to determine mitochondrial morphology. Bar = 10 µm. Left panel- bar graphs of the mitochondrial length analysis. Data are expressed as MEAN ± SEM of five independent experiments ****p≤0.0001. Mann-Whitney test . (B) Upper panel- representative Western blot of PGC1α and GADPH as a loading control in HCT116 cells treated or not with postbiotic Pd. Bottom panel- Bar graphs represent quantification of PGC1α/GADPH expressed as MEAN ± SEM of five independent experiments. ∗p < 0.05. Mann-Whitney test . (C) Upper panel- representative Seahorse trace of HCT116 cells treated or not with postbiotic Pd. A; oligomycin (1 µM), B;FCCP (250 µM), C; rotenone plus antimycin A (1 µM each). Bottom panel- basal and maximum OCR of HCT116 cells treated or not with postbiotic Pd. MEAN ± SEM of three independent experiments with 10 replicates each. ***P < 0.001 compared to control. Mann-Whitney test. (D) Upper panel- representative Western blot of CHOP and β-actin as a loading control in HCT116 cells treated or not with postbiotic Pd. Bottom panel- bar graphs represent quantification of CHOP/β-actin expressed as MEAN ± SEM of 3 independent experiments. ∗p < 0.05. Mann-Whitney test . (E) Upper panel- representative Western blot of VDAC1 and β-actin as a loading control in colon mucosa samples from 26-months-old mice treated or not with postbiotic Pd. Bottom panel- bar graphs represent quantification of VDAC1/β-actin expressed as MEAN ± SEM. Note that β-actin blot in is the same as in this figure, as the same membrane was stripped and re-probed for VDAC1. N = 3 in the control group and 4 for the Pd treated group. ∗p < 0.05. Mann-Whitney test .

Article Snippet: Human colorectal cell line HCT116 (ATCC) was maintained at 37oC (95%/5% air/CO 2 ) in DMEM media (GIBCO) supplemented with 10% (v/v) FBS.

Techniques: Labeling, MANN-WHITNEY, Western Blot, Control, Membrane

Journal: Light, Science & Applications

Article Title: Helical opto-thermoviscous flows drive out-of-plane rotation and particle spinning in a highly viscous micro-environment

doi: 10.1038/s41377-026-02303-8

Figure Lengend Snippet: a TVF-driven reorientation of a dimer structure to align its original axis of symmetry with the x – y imaging plane, significantly enhancing the visibility of the interparticle gap and highlighting advantages over conventional 3D confocal microscopy in resolving specific features. b Orientation-resolved 2D imaging of a self-assembled trimer around different rotational axes. c Schematic overview of the controlled release and rotation of substrate-anchored microstructures fabricated via two-photon lithography, illustrating the robustness and flexibility of the helical TVFs. d Out-of-plane rotation of a single micro-tile about different rotational axes, whose surfaces exhibit engraved patterns. e Out-of-plane rotation of a yeast cell, enabling visualization of the hidden bud. f Multi-view microscopy for high-resolution 3D imaging of HCT116 cells. While an original image stack did not resolve two cell nuclei even after digital rotation into the x – y plane, TVF rotation into plane allows the gap between the nuclei to be clearly resolved. After multi-view image fusion (lower row), this gap can be observed from any axial orientation. The scale bars are 5 μm

Article Snippet: The HCT116 cells (ATCC, CCL-247) were cultured in DMEM + GlutaMAX (Gibco) supplemented with 10% FBS (Gibco) and 1% penicillin-streptomycin (Gibco) at 37 °C and 5% CO 2 .

Techniques: Imaging, Confocal Microscopy, Microscopy, 3D Imaging

Journal: Foods

Article Title: 10-Hydroxy-2-decenoic Acid Suppresses Colorectal Cancer Progression by Inhibiting Wnt/β-Catenin Signaling and Promoting Apoptosis

doi: 10.3390/foods15091608

Figure Lengend Snippet: 10-HDA inhibited CRC cell growth and gap closure and increased intracellular ROS. ( A ) MTT analysis of HCT 116 and HT-29 cell viability after 48 h exposure to 10-HDA. ( B ) Colony formation of HCT 116 cells following 14 d treatment with 10-HDA. ( C ) Scratch assay analysis of HCT 116 gap closure after 24 h incubation with 10-HDA. ( D ) Flow cytometric analysis of HCT 116 intracellular ROS levels following 3 h exposure to 10-HDA. ** p < 0.01, *** p < 0.001, **** p < 0.0001; ns, no statistical significance. Control, vehicle-treated cells.

Article Snippet: Human colorectal carcinoma cell lines HCT 116 (ATCC; CCL-247) and HT-29 (ATCC; HTB-38) were purchased from Zhong Qiao Xin Zhou Biotechnology Co., Ltd. (Shanghai, China).

Techniques: Wound Healing Assay, Incubation, Control

Journal: Foods

Article Title: 10-Hydroxy-2-decenoic Acid Suppresses Colorectal Cancer Progression by Inhibiting Wnt/β-Catenin Signaling and Promoting Apoptosis

doi: 10.3390/foods15091608

Figure Lengend Snippet: 10-HDA inhibited tumor growth and modified the histopathological features of HCT 116 xenografts in vivo. ( A ) Schematic overview of HCT 116 xenograft establishment and 10-HDA administration. ( B ) Representative images of xenograft tumors from saline-treated control mice and 10-HDA-treated mice, with corresponding tumor volume quantification. Control, blue; 100 mg/kg 10-HDA, orange; 200 mg/kg 10-HDA, purple. ( C ) H&E-stained sections of xenograft tumors. Red arrows indicate tumor cells; yellow arrows denote necrotic regions; blue arrows indicate atypical (pathological) mitotic figures; and green arrows indicate inflammatory cell infiltration. ( D ) Ki-67 immunohistochemical staining of xenograft tumors from control and 10-HDA-treated mice. n = 6; * p < 0.05, ** p < 0.01, **** p < 0.0001; ns, no statistical significance. Control, saline-treated tumor-bearing mice.

Article Snippet: Human colorectal carcinoma cell lines HCT 116 (ATCC; CCL-247) and HT-29 (ATCC; HTB-38) were purchased from Zhong Qiao Xin Zhou Biotechnology Co., Ltd. (Shanghai, China).

Techniques: Modification, In Vivo, Saline, Control, Staining, Immunohistochemical staining

Journal: Foods

Article Title: 10-Hydroxy-2-decenoic Acid Suppresses Colorectal Cancer Progression by Inhibiting Wnt/β-Catenin Signaling and Promoting Apoptosis

doi: 10.3390/foods15091608

Figure Lengend Snippet: Evaluation of the safety profile of 10-HDA treatment. ( A ) Mice weight changes compared with normal mice. Normal, red; model, blue; 100 mg/kg 10-HDA, orange; 200 mg/kg 10-HDA, purple. ( B ) Serum levels of liver function markers (AST, ALT, and ALP). ( C ) Representative H&E-stained histopathological sections of the heart, liver, spleen, lung and kidney. All analyses were performed on samples from healthy mice (normal control), and saline (model)- and 10-HDA-treated mice bearing HCT 116 xenograft tumors. n = 6; * p < 0.05, ** p < 0.01; ns, no statistical significance.

Article Snippet: Human colorectal carcinoma cell lines HCT 116 (ATCC; CCL-247) and HT-29 (ATCC; HTB-38) were purchased from Zhong Qiao Xin Zhou Biotechnology Co., Ltd. (Shanghai, China).

Techniques: Staining, Control, Saline

Journal: Foods

Article Title: 10-Hydroxy-2-decenoic Acid Suppresses Colorectal Cancer Progression by Inhibiting Wnt/β-Catenin Signaling and Promoting Apoptosis

doi: 10.3390/foods15091608

Figure Lengend Snippet: Transcriptomic analysis of ectopic xenograft tumor tissues derived from HCT 116 cells in 10-HDA- and saline-treated (control) mice. ( A ) Volcano plot of DEGs. Each dot represents one gene; red indicates significantly upregulated genes and green indicates significantly downregulated genes (FDR < 0.05 and |log2FoldChange| > 1). ( B ) Heatmap of representative DEGs related to tumor progression. Colors indicate relative expression (red, upregulation; blue, downregulation). ( C ) GO enrichment of DEGs showing top terms in biological process (BP, red), cellular component (CC, green), and molecular function (MF, blue). ( D ) Top 20 enriched KEGG pathways of DEGs. The x-axis shows the number of DEGs in each pathway; bar color represents statistical significance (adjusted p -value). ( E ) Relative mRNA expression levels of ANO1 , MKi67 , LTBP1 , FGF19 , NECTIN4 , PLD1 , and SKA1 in HCT 116 cells treated with 10-HDA or vehicle (control). Control, dark blue; 1.0 mM 10-HDA, blue; 1.5 mM 10-HDA, light blue; 2.0 mM 10-HDA, green. ( F ) Relative mRNA expression levels of ANO1 , MKi67 , LTBP1 , FGF19 , NECTIN4 , PLD1 , and SKA1 in xenograft tumors from 10-HDA- or saline-treated (control) mice. Control, green; 100 mg/kg 10-HDA, blue; 200 mg/kg 10-HDA, yellow. * p < 0.05, ** p < 0.01, **** p < 0.0001; ns, no statistical significance.

Article Snippet: Human colorectal carcinoma cell lines HCT 116 (ATCC; CCL-247) and HT-29 (ATCC; HTB-38) were purchased from Zhong Qiao Xin Zhou Biotechnology Co., Ltd. (Shanghai, China).

Techniques: Derivative Assay, Saline, Control, Expressing

Journal: Foods

Article Title: 10-Hydroxy-2-decenoic Acid Suppresses Colorectal Cancer Progression by Inhibiting Wnt/β-Catenin Signaling and Promoting Apoptosis

doi: 10.3390/foods15091608

Figure Lengend Snippet: 10-HDA induced apoptosis in HCT 116 cells and subcutaneous HCT 116 xenograft tumors. ( A ) Relative mRNA expression levels of Bcl-2 , BAX , BINP2 , and caspase-3 in HCT 116 cells treated with 10-HDA or vehicle (control). Control, dark blue, 1.0 mM 10-HDA, blue; 1.5 mM 10-HDA, light blue; 2.0 mM 10-HDA, green. ( B ) Relative mRNA expression levels of Bcl-2 , BAX , BINP2 , and caspase-3 in xenograft tumors from 10-HDA- or saline-treated (control) mice. Control, green; 100 mg/kg 10-HDA, blue; 200 mg/kg 10-HDA, yellow. ( C ) Western blotting analysis of caspase-3, Bcl-2, BAX, and cleaved caspase-3 protein levels, as well as the calculated cleaved caspase-3/caspase-3 and Bcl-2/BAX ratios in HCT 116 cells treated with 10-HDA or vehicle (control). ( D ) Immunohistochemical staining of Bcl-2 and BAX in xenograft tumors from 10-HDA- or saline-treated (control) mice. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001; ns, no statistical significance.

Article Snippet: Human colorectal carcinoma cell lines HCT 116 (ATCC; CCL-247) and HT-29 (ATCC; HTB-38) were purchased from Zhong Qiao Xin Zhou Biotechnology Co., Ltd. (Shanghai, China).

Techniques: Expressing, Control, Saline, Western Blot, Immunohistochemical staining, Staining

Journal: Foods

Article Title: 10-Hydroxy-2-decenoic Acid Suppresses Colorectal Cancer Progression by Inhibiting Wnt/β-Catenin Signaling and Promoting Apoptosis

doi: 10.3390/foods15091608

Figure Lengend Snippet: Involvement of Wnt/β-Catenin signaling in the anti-CRC effects of 10-HDA. ( A ) Relative mRNA expression levels of WNT11 , RNF43 , FRAT1 , β-catenin , and MMP7 in HCT 116 cells treated with 10-HDA or vehicle (control). Control, dark blue; 1.0 mM 10-HDA, blue, 1.5 mM 10-HDA, light blue; 2.0 mM 10-HDA, green. ( B ) Relative mRNA expression levels of WNT11 , RNF43 , FRAT1 , β-catenin , and MMP7 in xenograft tumors from 10-HDA- or saline-treated (control) mice. Control, green; 100 mg/kg 10-HDA, blue; 200 mg/kg 10-HDA, yellow. ( C ) Western blotting analysis of β-catenin expression in HCT 116 cells treated with 10-HDA or vehicle (control). ( D ) Western blotting analysis of β-catenin, p-GSK3β and GSK3β in xenograft tumor tissues from 10-HDA- and saline-treated (control) mice. ( E ) Immunohistochemical staining of β-catenin in xenograft tumors. ( F ) Western blotting analysis and densitometric quantification of the nuclear and cytoplasmic β-catenin in HCT 116 cells after 10-HDA or vehicle (control) treatment. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001; ns, no statistical significance.

Article Snippet: Human colorectal carcinoma cell lines HCT 116 (ATCC; CCL-247) and HT-29 (ATCC; HTB-38) were purchased from Zhong Qiao Xin Zhou Biotechnology Co., Ltd. (Shanghai, China).

Techniques: Expressing, Control, Saline, Western Blot, Immunohistochemical staining, Staining

Journal: Cancer Biology & Therapy

Article Title: MBD2 suppresses SFRP1 expression and promotes colorectal cancer development by blocking MED19 binding to its methylated promoter

doi: 10.1080/15384047.2026.2667568

Figure Lengend Snippet: Inhibition of MBD2 upregulated the expression of SFRP1 and inhibited Wnt signaling pathway activation. (A and B) The interference efficiency of MBD2 siRNAs at the protein level in SW480 and HCT116 cells. (C) Silencing MBD2 increased the mRNA level of SFRP1. (D and E) Silencing MBD2 increased the protein level of SFRP1 and decreased the protein level of β -catenin. (F) KCC07 disrupted MBD2 binding to the SFRP1 promoter. (G and H) The half inhibitory concentration (IC50) of KCC07 in CRC cells. (I) KCC07 increased the mRNA level of SFRP1. (J and K) KCC07 increased the protein level of SFRP1 and decreased the protein level of β -catenin. n = 6, *indicates p < 0.05, **indicates p < 0.01.

Article Snippet: Normal colon mucosa cell line NCM460 (ATCC, CRL-1642 TM ) and the human colorectal cancer cell lines HCT116 (ATCC, CCL-247 TM ) and SW480 (ATCC, CCL-228 TM ) were purchased from iCell, and all had STR identification reports.

Techniques: Inhibition, Expressing, Activation Assay, Binding Assay, Concentration Assay

Journal: Cancer Biology & Therapy

Article Title: MBD2 suppresses SFRP1 expression and promotes colorectal cancer development by blocking MED19 binding to its methylated promoter

doi: 10.1080/15384047.2026.2667568

Figure Lengend Snippet: The effect of KCC07 on recovering SFRP1 expression requires MED19. (A and B) The interfering efficiency of MED19 siRNAs at the protein level in SW480 and HCT116 cells. (C–F) Treatment with KCC07 resulted in increased SFRP1 expression and increased β -catenin expression at both the mRNA and protein levels in CRC cells, while simultaneous knockdown of MED19 abolished these effects. n = 6, ns indicates not significant, *indicates p < 0.05, and **indicates p < 0.01.

Article Snippet: Normal colon mucosa cell line NCM460 (ATCC, CRL-1642 TM ) and the human colorectal cancer cell lines HCT116 (ATCC, CCL-247 TM ) and SW480 (ATCC, CCL-228 TM ) were purchased from iCell, and all had STR identification reports.

Techniques: Expressing, Knockdown

Journal: RSC Advances

Article Title: Synthesis, structural characterization and molecular docking analysis of novel β-ketoiminato palladium( ii ) complexes with anticancer properties

doi: 10.1039/d5ra09325b

Figure Lengend Snippet: Combined histogram showing the IC 50 values of the Pd( ii ) complexes 4 and 5 against the MCF-7 and HCT-116 cell lines.

Article Snippet: Cancer cell lines were obtained from the ATCC (Manassas, VA), MCF7 human breast cancer cell line (ATCC® HTB-22TM, USA), HCT-116 colon cancer cell line (ATCC CCL-247TM, USA) and human skin fibroblast (ATCC® PCS-201-012TM).

Techniques:

Journal: RSC Advances

Article Title: Synthesis, structural characterization and molecular docking analysis of novel β-ketoiminato palladium( ii ) complexes with anticancer properties

doi: 10.1039/d5ra09325b

Figure Lengend Snippet: Combined histogram displaying the binding energy scores of the Pd( ii ) complexes 4 and 5 against the KRAS-G13D, PIK3CA-H1047R and ATM-A112V targeted proteins in HCT-116.

Article Snippet: Cancer cell lines were obtained from the ATCC (Manassas, VA), MCF7 human breast cancer cell line (ATCC® HTB-22TM, USA), HCT-116 colon cancer cell line (ATCC CCL-247TM, USA) and human skin fibroblast (ATCC® PCS-201-012TM).

Techniques: Binding Assay